Kinda depends on which steering setup you have on your 01, they change them half way through that year. You should re-adjust it if you have the steering box on the axle, if it's on the frame your probably about right. Tire size isn't all that important, you'd adjust both 18" or 22" the same way.
If you have a 95-2001.5 need to have your cart sitting on the ground (not jacked up) preferably with someone in it when doing the adjustment. They're suppose to have toe-in, especially carts with the steering box mounted to the frame. they like to dart from side to side if not adjusted right.
Take a look at the Technician's service and repair manual electric carts
http://www.buggiesgonewild.com/elect...g-manuals.html
page G27.
If your cart is a 2001.5 and later with the steering box on the axle then you don't need as much toe-in, calls for 1/8" on these. It's listed at the very first of section G